Help Oreo Beat Osteosarcoma

Hello everyone,

My name is Antonio, and I am here to seek help for my beloved dog, Oreo. Oreo is a beautiful 1.5-year-old merle Border Collie, full of energy and love. Unfortunately, he has recently been diagnosed with osteosarcoma, a very aggressive type of bone cancer.

His story:

Oreo is my very first pet, and I love him so much. Since he came into my life, he has been a constant source of joy and happiness. As I am living alone, Oreo's companionship means the world to me. He came from a pack of dogs that his previous owners didn't want, and he was covered in ticks and fleas. I did my best to take care of him during his first few months. His playful spirit and unconditional love have been my greatest comfort in both good times and bad. He is always there to lift my spirits with his playful antics and constant companionship.

What's going on:

The diagnosis of osteosarcoma has been devastating for me and Oreo. This type of cancer requires immediate and costly treatment, including surgery, chemotherapy, and continuous care. I want to give Oreo the best possible chance to fight this disease so he can continue enjoying a life full of love and play.

How You can help:

I am launching this GoFundMe campaign to raise funds for Oreo's treatment. I heartbroken by his diagnosis and want to give him the best chance to fight this disease. Any contribution, big or small, will be greatly appreciated and will bring us one step closer to giving Oreo the chance he deserves. The funds raised will go towards:

Your support will help Oreo continue to be the joyful, playful companion who has brought so much happiness into my life.

My Eternal Gratitude

I am deeply grateful for any donation you can make. If you are unable to contribute financially, I would appreciate it if you could share this campaign with your friends and family to help us reach more people.

Oreo is more than just a pet; he is my first and only pet, my loyal companion, and my source of joy in a life I live alone. The thought of losing him to this cruel disease is heart-wrenching. I am willing to do everything possible to save his life. With your help, we can give Oreo the chance to fight and overcome this devastating illness.

Thank you for taking the time to read our story and consider helping Oreo.

With all my gratitude,

Antonio.

Edit: Results from clinic.

### Translation of the Document for Oreo

#### Page 1 of 4

Date of Admission: 14/05/2024

ID Number: 426814

Microchip: 94101----(removed for privacy)

Date of Extraction:

Patient: "OREO", 16411

Species: DOG

Source: (1558) CV ALQUIAN

Technique Result Reference Value Low Normal High

PATHOLOGICAL ANATOMY

CIF: B-19579085

c/ Tucumán 6 Nave B, 18200 MARACENA (Granada)

Authorized agro-livestock laboratory with number 18/05/PR/PSA

Granada, 15/05/2024

José Sánchez

Technical Director

#### Page 2 of 4

Date of Admission: 14/05/2024

ID Number: 426814

Microchip: 94101----(removed for privacy)

Date of Extraction:

Patient: "OREO", 16411

Species: DOG

Source: (1558) CV ALQUIAN

Technique Result Reference Value Low Normal High

PATHOLOGICAL ANATOMY

GENERAL CYTOLOGY REPORT

Dog with osteolytic lesion in the right scapula. Samples were taken using fine needle aspiration.

Four stained samples were received (apparently fast protocol Romanowsky type aqueous) and four unstained fixed samples (processed with Wright-Giemsa protocol). Samples were dehydrated and mounted in acrylic medium prior to evaluation.

#### CYTOLOGICAL EXAMINATION

All samples show similar findings, with moderate cellularity and common monolayers (much rarer non-evaluable multilayers).

Only Wright-Giemsa stained smears were used for evaluating atypia criteria.

Background shows occasional mineralized cellular debris (markedly basophilic) and occasional to rare organized acidophilic collagen matrix deposits compatible with osteoid. Rare corneal scales and adipocytes (contamination from the skin) are observed. Occasional blood capillaries (typical morphology) and more common collagen fiber aggregates (occasionally in apposition with osteoid deposits) are detected.

Blood contamination is marked, with large platelet aggregates. No pathological signs or activation (absence of inflammatory signs to identify an inflammatory-infectious process, so blood contamination is more likely) were detected in the rare leukocytes present in the sample.

Excluding blood contamination leukocytes and previously mentioned contamination cells, most cells correspond to atypical mesenchymal cells. Mesenchymal cells show oval to polygonal morphology, commonly isolated and rarely in groups or clusters of 5-10 cells. No intercellular junction images were observed in these groups, nor were acinar, tubular, or other epithelial subtype conformations detected. Rarely, these clusters correlate with very loose collagen fiber deposits (however, no correlation with osteoid deposits or clear relation with blood capillaries was observed).

These cells are usually medium to large, with diameters between 2.5 to 4.5 erythrocytes. The cytoplasm is moderate to abundant, with defined borders and dark bluish coloring, with rare rounded perinuclear clearing. No pigments are observed in these cytoplasms, but small perinuclear vacuole collections are common. Also observed (though more rarely) is minute to very fine markedly acidophilic granulation (randomly arranged, no tendency to coalesce). The nuclear region usually appears peripheral, being round to oval, sometimes indented, and large (between 2.5 to 3.5 erythrocytes in diameter). Binucleation (average 2.5 per 10 fields at 400x magnification) and multinucleated cells (with common intracellular anisokaryosis, average 2 per 10 fields at 400x magnification) are common. Chromatin is reticular, markedly detailed, and nucleoli (usually 1 or 2), large and variously shaped, are common. No mitosis images are detected.

Among the atypia markers observed in the described cells, the following stand out:

- Marked anisocytosis.

- Marked anisokaryosis.

- Marked anisonucleolosis.

- Common atypical nucleoli and severely detailed chromatin images.

- Marked karyomegaly.

- Common binucleated and multinucleated cells.

Osteoclasts (typical but markedly reactive) are occasionally noted. No typical pathogens were detected in the submitted samples (with the performed stains).

#### DIAGNOSIS

Cytologically compatible with osteosarcoma, see comment.

#### COMMENT

Cytological findings in the evaluated sample are consistent with a malignant mesenchymal neoplasm with osteoblastic differentiation, with the most compatible morphotype being osteosarcoma (although certain intracellular morphological details could correspond to chondrosarcoma or chondroblastic osteosarcoma, the absence of a typical chondroid neoplasia background led to prioritizing the osteosarcoma diagnosis). The appearance of relatively high exfoliation and other mesenchymal atypia criteria make it unlikely that the lesion is a benign bone neoplasm or secondary osteolytic lesion (all these findings being more compatible with typical cytological descriptions of osteosarcoma). The main differential would be another sarcoma subtype (fibrosarcoma, hemangiosarcoma, histiocytic sarcoma, etc.) with bone involvement or osteoblastic differentiation. It is advised to combine cytological results with other tests (imaging, history, etc.).

#### NOTE

Cytological techniques should be understood as diagnostic support and their assessment should be performed in conjunction with other complementary tests to reach a final diagnosis. Sensitivity and specificity of cytology depend on the studied tissue, always subject to proper initial sampling. Ultimately, consult your veterinarian for appropriate interpretation of this report.

Alejandro Pérez-Écija DVM, PhD, MSc, Diplomate of the European College of Veterinary Pathologists (ECVP).

Cytology validated by Alejandro Pérez-Écija.

CIF: B-19579085

c/ Tucumán 6 Nave B, 18200 MARACENA (Granada)

Authorized agro-livestock laboratory with number 18/05/PR/PSA

Granada, 15/05/2024

José Sánchez

Technical Director

-------------------

Edit 20/05/2024.

I increased the GoFundMe goal by €500. The surgery has been paid for, and hopefully, the medical bills for the chemo sessions and post-operative visits will be covered. If you would like to contribute, any additional donations will now go towards making Oreo's recovery as comfortable and joyful as possible. This includes toys, food, a new (and fluffier) bed, treats, bones, vet visits, and more. It's all about spoiling him as the good boy he is.